Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences TrizmaR base Primary Stand25KG
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations such as Tris-EDTA (TE) buffer Tris-acetate-EDTA (TAE) buffer Tris-borate-EDTA (TBE) buffer etc. It is pure essentially stable relatively non-hygroscopic and has a high equivalent weight.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959188 ELUTE BUFFER B 50MM TRIS HCL8
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Medchemexpress LLC PIPES dipotassium | 108321-27-3 | MFCD00069751 | 98.0% | 378.55 | C8H16K2N2O6S2 | 10 G
PIPES dipotassium is a zwitterionic buffering agent used in biochemical and biological assays to maintain a stable, near-neutral pH. The dipotassium salt provides good aqueous solubility and is compatible with many enzymatic and cell-based applications.
- Maintains pH in the 6.1-7.5 buffering range.
- Zwitterionic structure minimizes metal binding and enzymatic interference.
- High purity (98.0%).
- White to off-white solid, water soluble.
- Available in 10 G and larger lab-scale pack sizes.
- Store at room temperature; keep under nitrogen and away from moisture; in solution store at -20°C to -80°C for long-term storage.
- Supplied with SDS and COA documentation.
Gold Biotechnology Inc MES Free Acid Monohydrate
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
United States Biological Corporation Tris Base Ultrapure, 77-86-1, MFCD00004679, 5kg
Molecular Formula C₄H₁₁NO₃, Purity ≥99.9%, Molecular Biology Grade, Molecular Weight 121.14, Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity.
Rockland Immunochemicals Normal Saline - MB-049-1000
This solution is commonly referred to as physiological saline or isotonic saline.
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 500ML
HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 500ML
Medchemexpress LLC BES sodium | 66992-27-6 | 97.0% | 25 G
BES sodium is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is used in the diagnostic testing area.
- Biochemical buffer
- Effective pH range: 6.4 to 7.8 (pKa: 7.15 at 20 °C)
- Used in diagnostic testing
- Appearance: Solid
- Color: White to off-white
- Target: Biochemical assay reagents
- Pathway: Others
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.95% | 250 MG
MES monohydrate is an analytical standard intended for research and analytical applications. It is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments as a reference standard for assays.
- Used in cell culture
- Used in enzyme activity determination
- Used in electrophoresis
- Used in protein studies
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS
eMolecules Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4